Lethal Atlantic salmon virus found on the West Coast

Tonington

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More details on the absolutely pathetic denial/ass covering as manifest by the servants of mammon/aquaculture feedlots aka DFO.

Positively Negative - How the CFIA Failed to Defuse ISA in BC | Super Heroes 4 Salmon

Positive PCR results are not enough to confirm the presence of a virus...you guys are out of your depth here. Since pathogens can share many genes, a positive PCR result doesn't actually mean that there was ISA virus in the sample. The polymerase chain reaction can be very powerful for detecting low amounts of virus in a sample, but what DNA does get amplified is entirely dependent on the binders in the test. So, when a virus shares a piece of DNA with other virins...well that means that the PCR that binds for that gene, is not highly specific at all ...the OIE recognizes this, and requires clinical disease symptoms, or isolation of the virus in cell culture to confirm PCR positive tests.

The fish weren't showing clinical signs of ISA, and they have yet to grow any of the virus in cell factories.

The positive result from Norway was 1 out of a few more than 30 tests of the same sample. That's not enough to confirm the presence of the virus.

Spin spin spin....
 

taxslave

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Positive PCR results are not enough to confirm the presence of a virus...you guys are out of your depth here. Since pathogens can share many genes, a positive PCR result doesn't actually mean that there was ISA virus in the sample. The polymerase chain reaction can be very powerful for detecting low amounts of virus in a sample, but what DNA does get amplified is entirely dependent on the binders in the test. So, when a virus shares a piece of DNA with other virins...well that means that the PCR that binds for that gene, is not highly specific at all ...the OIE recognizes this, and requires clinical disease symptoms, or isolation of the virus in cell culture to confirm PCR positive tests.

The fish weren't showing clinical signs of ISA, and they have yet to grow any of the virus in cell factories.

The positive result from Norway was 1 out of a few more than 30 tests of the same sample. That's not enough to confirm the presence of the virus.

Spin spin spin....

When their source of "information" is Susuki and Alexandra Morton(no relation thankyou verymuch) they don't have to understand the science just push the repeat button. The chicken little syndrome works well when one is short on facts.
 

Tonington

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We use PCR at work....but we also have obvious symptoms of clinical disease, whether it's gross pathology, or organ histology. If PCR is not enough in our case for confirmed loss, then it's certainly not enough for this case.

It's ludicrous in the extreme, as well as completely ignorant about the type of testing that needs to be done to say that the tests thus far have confirmed the presence of the virus. Also, I'm highly skeptical about the quote that blog said they had from Nyland:
"Our results are not conclusive, but do suggest ... that an ISA virus is present in wild populations of O. nerka (Pacific sockeye)," Dr. Are Nylund, a professor of biology at the University of Bergen, wrote in an email exchange with The Seattle Times.
Is that an elipsis or does Nyland use consecutive dots like I do...hmm.

The chain of custody is definitely suspect. DNA results can be thrown out of court when chain of custody is out of spec. For good reason.
 

bill barilko

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Speaking of spin maybe you could tell us how ISA devastated the Chilean Salmon feedlots so completely that they had to up and move 200 miles further south into unpolluted Patagonian wilderness waters-and have only now started dumping more finished crud on the international marketplace and further depressing prices.

So have any of you geniuses actually eaten any of that sh!t lately?

Curious (but not too curious) mind wants to know.
 

Tonington

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Speaking of spin maybe you could tell us how ISA devastated the Chilean Salmon feedlots so completely that they had to up and move 200 miles further south into unpolluted Patagonian wilderness waters-and have only now started dumping more finished crud on the international marketplace and further depressing prices.

Well, they moved infected eggs into Chile, and now they have ISA in Chile. The disease was confirmed by large mortality events, clinically diseased fish, and PCR.

That situation has no relationship to the situation in BC. If it was in BC waters, there would be dead fish.

So yes, that was spin on your part. As per usual.
 

bill barilko

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Well, they moved infected eggs into Chile, and now they have ISA in Chile. The disease was confirmed by large mortality events, clinically diseased fish, and PCR. That situation has no relationship to the situation in BC. If it was in BC waters, there would be dead fish.
Die offs in the BC feedlots are documented-worse to come it's only a matter of time.

I was talking last night with another sportfisherman/conservationist about the issue with Orcas eating Chinook Salmon and proposed restrictions to come and he remarked on how Orcas never frequent the areas around net pens-in fact they generally avoid them like the plague-or like they are a plague take your pick.

Much smarter than Seals are Orcas-Seals around net pens usually end up being fed hot lead.

Funny how a place that purports to be all about raising live animals is in fact a pit of disease and death.
 

Tonington

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Die offs in the BC feedlots are documented-worse to come it's only a matter of time.

ISA kills up to 90% of all fish in an infected net pen. That has not happened. Fish die all the time from natural causes. It's natural. They produce tens of thousands to millions of eggs for that very reason.
 

Tonington

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A funny coincidence, my third home assignment in a veterinary biostatistics course I'm taking right now from the AVC is on test specificity and sensitivity, for ISA! lol I just submitted it online to my prof.
 

Tonington

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A little bit late to the party, but another voice laying the bare facts out, though I wouldn't say no evidence of ISA in BC, I would say weak and suspect evidence :
There is no evidence of ISA in waters off B.C

By Robert Wager, The Daily News November 24, 2011

It never fails to amaze me how activist groups carry out "science."

When their poorly done "science" was slightly positive for ISA they had a press release and claimed imminent disaster for B.C. wild salmon. The story ran on every front page across North America.

Forget the fact ISA has virtually no effect on Pacific salmon species. Forget the fact DFO and other world-class experts have repeated the testing of the allegedly positive ISA salmon samples, found by a well known anti-aquaculture activist, and did not confirm evidence of the ISA virus. Forget the fact thousands of similar tests over the years have all come back negative for ISA. Forget the fact all salmon egg imports have been tested for ISA for years. Now the critics claim DFO is not concerned about ISA.

Are we to believe hundreds more proper controlled, replicated tests, with negative results, are because DFO is unconcerned? What an amazing point of view. It might keep the fear about ISA in the media for a little while longer but it sure isn't science.

The science is clear. There continues to be no evidence of the ISA virus in B.C. waters.

Robert Wager Vancouver Island University Nanaimo
There is no evidence of ISA in waters off B.C

WTH?

What stocks are those?

Seriously? WTH?



I don't know about you but I see a large portion of that distribution is outside Canadian waters...

Gold Seal - Salmon Migration Pattern - Salmon of the High Sea
 

Tonington

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'Pay no attention to that man behind the curtain'.

In clinical disease challenges, we can't kill Oncorhynchus sp. with ISAV, nor can we induce clinical symptoms of disease. Your activist rhetoric won't change that.
 

Tonington

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This Just In-Viruses Mutate that's just one problem with the slippery little bastids.

No s h i t Sherlock. Doesn't change the fact that ISA doesn't cause disease in any Oncorhynchus species. Doesn't mean it ever will either.

The Swimming Dead



ZOMBIE SALMON OVER RUN RIVERS

That looks to be similar in quality to the samples Alexandra Morton was collecting and storing in her freezer. Activist's don't seem to care much about sample quality or integrity...