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quandary121
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lincolnshire
  #1
Jul 1st, 2008
URGENT WARNING: H5N1 DNA in Flu Vaccine


avian flu
what are they up too millions of people in britain are given the anti flu jab each year especially the old folks, Ive always been suspicious of it, and this confirms why there is a good reason to be.!!!
Flu Mist a popular over the counter live flu vaccine has been found to contain two H5N1 DNA markers, these markers seem to have been sewn into the flu mist vaccine to ensure that H5N1 or another strain of Avian Flu goes pandemic.
This is going to cause major problems before long, it is imperative that people are warned about flu mist.
Tonington
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  #2
Jul 1st, 2008
Do you actually know how a vaccine works? H5N1 is a broad subclass of the Influenza A virus, of which the bird flu (HPAI A(H5N1) belongs. The H5N1 just refers to the protein structures on the viral envelope, so a broad spectrum vaccine can be produced by adding the deactivated proteins to the vaccine. Then your immune system can begin to build the antibodies by recognition of the antigen.

This is a preventative measure. Vaccines are the sole reason we have eradicated one disease, a rather famous one really. Smallpox.
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quandary121
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  #3
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Quoting Tonington
Do you actually know how a vaccine works? H5N1 is a broad subclass of the Influenza A virus, of which the bird flu (HPAI A(H5N1) belongs. The H5N1 just refers to the protein structures on the viral envelope, so a broad spectrum vaccine can be produced by adding the deactivated proteins to the vaccine. Then your immune system can begin to build the antibodies by recognition of the antigen.

This is a preventative measure. Vaccines are the sole reason we have eradicated one disease, a rather famous one really. Smallpox.
Quote:
A pathogen (from Greek pathos, suffering/emotion, and gene, to give birth to), infectious agent, or more commonly Please register to see links, is a Please register to see links that causes Please register to see links or Please register to see links to its Please register to see links.Please register to see links The term pathogen is derived from the Please register to see links "that which produces suffering." There are several substrates and pathways whereby pathogens can invade a host; the principal pathways have different episodic time frames, but Please register to see links has the longest or most persistent potential for harboring a pathogen.
The body contains many natural defenses against some of the common pathogens (such as Please register to see links) in the form of the human Please register to see links and by some "helpful" Please register to see links present in the human body's Please register to see links. However, if the immune system or "good" bacteria is damaged in any way (such as by Please register to see links, Please register to see links (HIV), or Please register to see links being taken to kill other pathogens), pathogenic Please register to see links that were being held at bay can proliferate and cause harm to the host. Such cases are called Please register to see links.
Some pathogens (such as the bacterium Please register to see links, which may have caused the Please register to see links, the Please register to see links virus, and the Please register to see links protozoa) have been responsible for massive numbers of casualties and have had numerous effects on afflicted groups. Of particular note in modern times is HIV, which is known to have infected several million humans globally, along with Please register to see links (SARS) and the Please register to see links virus. Today, while many medical advances have been made to safeguard against infection by pathogens, through the use of Please register to see links, Please register to see links, and Please register to see links, pathogens continue to threaten human life. Social advances such as Please register to see links, Please register to see links, and Please register to see links have reduced the threat from some pathogens.

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The global spread of highly pathogenic H5N1 in Please register to see links is considered a significant Please register to see links threat.
While other Please register to see links strains are known, they are significantly different from a current, highly pathogenic H5N1 strain on a genetic level, making the global spread of this new strain unprecedented. The H5N1 strain is a Please register to see links, highly Please register to see links Please register to see links virus (HPAI) found in multiple bird species. It is both epizootic (an epidemic in non-humans) and panzootic (a disease affecting animals of many species especially over a wide area). Unless otherwise indicated, "H5N1" in this article refers to the recent highly pathogenic strain of H5N1.
"Since Please register to see links, studies of H5N1 indicate that these viruses continue to evolve, with changes in antigenicity and internal Please register to see links constellations; an expanded host range in avian species and the ability to infect Please register to see links; enhanced pathogenicity in experimentally infected Please register to see links and ferrets, in which they cause systemic infections; and increased environmental stability."Please register to see links

Cumulate Human Cases of and Deaths from Please register to see links
As of Please register to see links, Please register to see linksPlease register to see linksNotes:
  • Source Please register to see links Please register to see links
  • "[T]he incidence of human cases peaked, in each of the three years in which cases have occurred, during the period roughly corresponding to winter and spring in the northern hemisphere. If this pattern continues, an upsurge in cases could be anticipated starting in late 2006 or early 2007." Please register to see links
  • The Please register to see links curve for deaths is y = a + ek x, and is shown extended through the end of April, 2007.
Tens of millions of birds have died of Please register to see links influenza and hundreds of millions of birds have been slaughtered and disposed of, to limit the spread of H5N1. Countries that have reported one or more major highly pathogenic H5N1 outbreaks in birds (causing at least thousands but in some cases millions of dead birds) are (in order of first outbreak occurrence): Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links.
Highly pathogenic H5N1 has been found in birds in the wild in numerous other countries: Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links. Surveillance of Please register to see links in humans, poultry, wild birds, cats and other animals remains very weak in many parts of Asia and Africa. Much remains unknown about the exact extent of its spread.
H5N1 has low pathogenic varieties Please register to see links in Please register to see links in North America. H5N1 has a highly pathogenic variety that is endemic in dozens of species of birds throughout south Please register to see links and is threatening to become endemic in birds in west Asia and Please register to see links. So far, it is very difficult for Please register to see links to become infected with H5N1. The presence of highly pathogenic (deadly) H5N1 around the world in both birds in the wild (Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, Please register to see links, etc.) and in Please register to see links and Please register to see links on farms has been demonstrated in millions of cases with the Please register to see links isolate actually Please register to see links in hundreds of cases yielding definitive proof of the Please register to see links of this strain of this subtype of the species Please register to see links (bird flu virus).
pathogenic H5N1
ARE YOU AWARE OF THE EFFECTS OF A PATHOGENIC VIRUS. THERE IS NO CURE FOR FLU. SO INTRODUCING A PATHOGENIC VIRUS, THAT IS CAPERBLE OF MUTATING INTO AN EVEN MORE VIRULENT STRAIN DOES NOT CONCERN YOU. OR THE FACT THAT THEY WILL INJECT IT INTO YOU, WHERE IT CAN OVER COME OR BODYS DEFENCES.
The global spread of highly pathogenic H5N1 in Please register to see links is considered a significant Please register to see links threat
quandary121
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  #4
Jul 1st, 2008
Quoting Tonington
Do you actually know how a vaccine works? H5N1 is a broad subclass of the Influenza A virus, of which the bird flu (HPAI A(H5N1) belongs. The H5N1 just refers to the protein structures on the viral envelope, so a broad spectrum vaccine can be produced by adding the deactivated proteins to the vaccine. Then your immune system can begin to build the antibodies by recognition of the antigen.

This is a preventative measure. Vaccines are the sole reason we have eradicated one disease, a rather famous one really. Smallpox.
While other Please register to see links strains are known, they are significantly different from a current, highly pathogenic H5N1 strain on a genetic level, making the global spread of this new strain unprecedented
Tonington
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  #5
Jul 1st, 2008
Yes I know what a pathogen is and I know what a pandemic is. I've taken microbiology courses.

You didn't answer my question. I take it you don't know how vaccines work.

You sound like those who said Jenner was crazy. He performed the first human trial on his kid, and now smallpox is extinct.

The virus mutates in hosts, and has to be pathogenic to do it. What you're talking about is a sub-unit vaccine, adding bits of genetic material, in this case the surface proteins. That is not a pathogen. In order for it to be pathogenic, it would need the genetic material that after entry to the cell(decapsulation):

a) directs the host cell to replicate it's own complete DNA
b) directs the host cell to reassemble it's DNA, while ignoring the host cells needs, inside a fully constructed viral envelope.
c) directs the host cell to lyse or form the envelope from cell glycoproteins when the new viruses bud out.

In order for that sequence to take place, you need all of the viral genetic material. It can't be done with just the genes which code for the protein structures on the envelope.
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  #6
Jul 1st, 2008
I think I have smallpox.
quandary121
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  #7
Jul 1st, 2008
Quoting Tonington
Yes I know what a pathogen is and I know what a pandemic is. I've taken microbiology courses.

You didn't answer my question. I take it you don't know how vaccines work.

You sound like those who said Jenner was crazy. He performed the first human trial on his kid, and now smallpox is extinct.

The virus mutates in hosts, and has to be pathogenic to do it. What you're talking about is a sub-unit vaccine, adding bits of genetic material, in this case the surface proteins. That is not a pathogen. In order for it to be pathogenic, it would need the genetic material that after entry to the cell(decapsulation):

a) directs the host cell to replicate it's own complete DNA
b) directs the host cell to reassemble it's DNA, while ignoring the host cells needs, inside a fully constructed viral envelope.
c) directs the host cell to lyse or form the envelope from cell glycoproteins when the new viruses bud out.

In order for that sequence to take place, you need all of the viral genetic material. It can't be done with just the genes which code for the protein structures on the envelope.
yes i know how vaccines work happy now.

This is not what is going on here though is it by introducing it into your body
BIG PROBLEM H5N1 DNA in Flu Vaccine people who have taken this will be coughing and sneezing this around for up to 3 weeks infecting others who have not had the Vaccine.
and without significant amount of vitamin c you will drown in your own blood from a scurvy like disease.yes or no
Tonington
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  #8
Jul 1st, 2008
Perhaps hypochondriasis Beaver?
Tonington
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  #9
Jul 1st, 2008
Quoting quandary121
This is not what is going on here though is it by introducing it into your body
BIG PROBLEM H5N1 DNA in Flu Vaccine people who have taken this will be coughing and sneezing this around for up to 3 weeks infecting others who have not had the Vaccine.
and without significant amount of vitamin c you will drown in your own blood from a scurvy like disease.yes or no
The answer is no.
darkbeaver
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  #10
Jul 1st, 2008
Quoting Tonington
Perhaps hypochondriasis Beaver?
You're probubly right, I'll sent you a bottle of pustules and you can confirm your diagnosis.
quandary121
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  #11
Jul 1st, 2008
Quoting Tonington
The answer is no.

ANTIGENIC DRIFT
ANTIGENIC SCHIFT
Avian Flu (2): Threat of a pandemic


Tonington
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  #12
Jul 1st, 2008
Quoting quandary121
ANTIGENIC DRIFT
ANTIGENIC SCHIFT
Avian Flu (2): Threat of a pandemic


And nowhere does he implicate vaccines.

You notice in your first video it explained that the new vaccine contains two of three required DNA packets? Requires, as in, won't work without.
quandary121
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  #13
Jul 1st, 2008
H1N1 IN 1918 KILLED 40 TO 50 MILLION PEOPLE AND WAS CALLED THE SPANISH FLU
H2N2 IN 1957 KILLED 2 MILLION PEOPLE AND WAS CALLED ASIAN FLU
H3N2 IN 1968 KILLED 1 MILLION PEOPLE AND WAS CALLED HONG KONG FLU

IT IS BELIEVED THAT IN ALL 3 CASES IT WENT FROM A BIRD THROUGH APIG THEN INTO A HUMAN

The Pentagon ordered $58 million of Tamiflu for troops. Then, Bush ordered states to buy over $500 million of Tamiflu. Tamiflu is manufactured and marketed by Roche, and Glead receives a 10% royalty. Rumsfeld served as chairman of Gilead before joining the Bush administration and still holds between $5 and 25 million in the company. He reportedly sold shares worth $5 million in 2005. Very profitable for him.
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  #14
Jul 1st, 2008
And that has what to do with MedImmune's Flu Mist or the safety of their vaccines?

Follow the advice in your signature...
quandary121
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  #15
Jul 1st, 2008
Quoting Tonington
And that has what to do with MedImmune's Flu Mist or the safety of their vaccines?

Follow the advice in your signature...

Flu Vaccine people who have taken this will be coughing and sneezing this around for up to 3 weeks infecting others who have not had the Vaccine.
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  #16
Jul 1st, 2008
The efficacy for flu vaccines like FLUMIST is about 85%. Getting a FLUMIST vaccine lessens those coughing and spreading the contagion. That's the whole point. Disease models are called SIR models, susceptible, infected and resistant. By vaccinating people, you reduce the susceptible portion of the curve by reducing transmission rates, and increasing recovery rates.
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  #17
Jul 1st, 2008
It's hopeless trying to explain anything about reality to quandary121, Tonington. He's got an impenetrable paranoid conspiracy theory about every subject he's ever raised or responded to.
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  #18
Jul 2nd, 2008
And I'm a glutton for punishment I guess. I have a hard time letting disinformation be put out there without doing my best to correct some of it. Wouldn't want some young and eager mind wandering the alleys on the net to be swayed by the dark side
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Understood, and I applaud your efforts. I do the same. The best we can hope for is that those young and eager minds will see the corrections and try to think seriously about who might be right, but we're never going to change the minds of the paranoid conspiracy theorists.
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  #20
Jul 2nd, 2008
Quoting Dexter Sinister
Understood, and I applaud your efforts. I do the same. The best we can hope for is that those young and eager minds will see the corrections and try to think seriously about who might be right, but we're never going to change the minds of the paranoid conspiracy theorists.
look dexter i would rather you did not reply to any of my threads, you seem to enjoy arguments, and trying to put me down in each and every one so do me a favor and F**k off OK, i don't need to discuss anything with your antiquated views, your total lack of knowledge of some of my subjects proves that you are a sad old git with to much time on your hands .

Quote:
Understood, and I applaud your efforts
This is how i expect you to answer anyone who disagrees with me over anything regardless of the facts, and if a qualified Dr is worried about the implications of H5N1 in flu jabs, i think that is worthy of debate, but with you i have found debate impossible, so if you haven't got the message that your not welcome to comment on my threads by now, i hope that you have got the message now
quandary121
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  #21
Jul 2nd, 2008
Quoting Tonington
The efficacy for flu vaccines like FLUMIST is about 85%. Getting a FLUMIST vaccine lessens those coughing and spreading the contagion. That's the whole point. Disease models are called SIR models, susceptible, infected and resistant. By vaccinating people, you reduce the susceptible portion of the curve by reducing transmission rates, and increasing recovery rates.
Quote:
The efficacy for flu vaccines like FLUMIST is about 85%
Do you think that this is a reasonable percentage.? considering the threat of a pandemic.! As even a slim chance is still something to be concidered worrying about or to be awear of ..
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Jul 2nd, 2008
85% is fantastic in the flu guessing game - considering in any year there may be a hundred or so brewing and labs have to squirrel it down to three or four.
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quandary121
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  #23
Jul 2nd, 2008
Quoting lone wolf
85% is fantastic in the flu guessing game - considering in any year there may be a hundred or so brewing and labs have to squirrel it down to three or four.
I STILL CONCIDER MY EARLIER COMENT TO BE VALID
considering the threat of a pandemic.! As even a slim chance is still something to be concidered worrying about or to be awear of ..

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to be awear of ..
better to be informed about someting then not told at all..!!!!
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  #24
Jul 2nd, 2008
Quoting quandary121
Do you think that this is a reasonable percentage.?
Yes, of course. Every year there is a new epidemic with the flu, which has the possibility of becoming a pandemic. It starts in Asia and moves east across the planet. So they develop vaccines based on what strain is moving out of Asia. 85% is a hell of a lot better than chancing it and having no vaccination program at all.
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Will this happen before the Mayan disaster, the world ending through planet X, chemtrails killing us all or is it part of the New World Order or the Illuminati plans? Do you have a conspiracy of the week plan or something that gets sent? And why shouldn't people question you, it's an open forum...
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quandary121
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  #26
Jul 2nd, 2008
Quoting Tonington
Yes, of course. Every year there is a new epidemic with the flu, which has the possibility of becoming a pandemic. It starts in Asia and moves east across the planet. So they develop vaccines based on what strain is moving out of Asia. 85% is a hell of a lot better than chancing it and having no vaccination program at all.

Quote:
85% is a hell of a lot better than chancing it and having no vaccination program at all
I'm not disputing this but don't you think that to engineer DNA in a flu vaccine is dangerous.????

Quote:
Amplification of influenza HA gene from RNA extracted directly from the tracheal aspirate obtained from the patient
Quote:
All human influenza virus HA genes characterized to date, including the 1918 viruses (Please register to see links), have a single, basic Arg residue at the cleavage site between HA1 and HA2. An insertion similar to the basic amino acid insertion adjacent to the cleavage site in the HA gene of the A/Hong Kong/156/97 virus has been found in several highly pathogenic avian H5 viruses. Basic amino acids adjacent to the cleavage site allow proteases other than trypsin-like proteases to cleave the HA into HA1 and HA2 domains. This event would enable the virus to spread systemically by altering the tissue range of these viruses from the respiratory and alimentary tract to other sites, such as the brain, heart, and blood vessels (Please register to see links). It has been suggested that the acquisition of a ubiquitously cleavable avian HA by a human influenza A virus could make a virus lethal (Please register to see links). It remains to be shown whether gene segments other than the HA may also have contributed to the ability of the virus to infect a human.


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A pandemic of influenza could begin with isolated cases, in which avian or swine influenza viruses adapt to human hosts or, over time, genetically reassort with circulating human influenza A viruses, or it could begin as a rapid and explosive spread of a pandemic virus derived from a reassortment event in an intermediate host. Increased surveillance efforts have been initiated to identify other cases of illness associated with influenza A (H5N1) viruses. In addition, serosurveys are under way in an effort to identify asymptomatic or mild clinical infections in the region and substantiate the previous report of seroprevalence to H5 viruses (Please register to see links). These studies may determine whether H5 viruses similar to A/Hong Kong/156/97 are circulating in the human population and if the isolation of the A/Hong Kong/156/97 virus is the first step in the recognition of an influenza A virus with pandemic potential or whether this case is simply an isolated event.
Note added in proof: Since the submission of this report, there have been 12 additional confirmed human cases of influenza A (H5N1) infections in Hong Kong, including three fatalities. Sequence analysis of the genes of six of the isolates revealed that all of the genes are of avian origin and are closely related to each other. The HA gene codes for a multiple basic amino acid insertion upstream of the cleavage site, associated with highly pathogenic avian influenza viruses and identical to that seen in the A/Hong Kong/156/97 virus.

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Science 16 January 1998:
Vol. 279. no. 5349, pp. 393 - 396
DOI: 10.1126/science.279.5349.393
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Reports


Characterization of an Avian Influenza A (H5N1) Virus Isolated from a Child with a Fatal Respiratory Illness


Kanta Subbarao, * Alexander Klimov, Jacqueline Katz, Helen Regnery, Wilina Lim, Henrietta Hall, Michael Perdue, David Swayne, Catherine Bender, Jing Huang, Mark Hemphill, Thomas Rowe, Michael Shaw, Xiyan Xu, Keiji Fukuda, Nancy Cox An avian H5N1 influenza A virus (A/Hong Kong/156/97) was isolated from a tracheal aspirate obtained from a 3-year-old child in Hong Kong with a fatal illness consistent with influenza. Serologic analysis indicated the presence of an H5 hemagglutinin. All eight RNA segments were derived from an avian influenza A virus. The hemagglutinin contained multiple basic amino acids adjacent to the cleavage site, a feature characteristic of highly pathogenic avian influenza A viruses. The virus caused 87.5 to 100 percent mortality in experimentally inoculated White Plymouth Rock and White Leghorn chickens. These results may have implications for global influenza surveillance and planning for pandemic influenza.

K. Subbarao, A. Klimov, J. Katz, H. Regnery, H. Hall, C. Bender, J. Huang, M. Hemphill, T. Rowe, M. Shaw, X. Xu, K. Fukuda, N. Cox, Influenza Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
W. Lim, Government Virus Unit, Queen Mary Hospital, Hong Kong, China.
M. Perdue and D. Swayne, Southeast Poultry Research Laboratory, Agriculture Research Service, U.S. Department of Agriculture, Athens, GA 30605, USA.
* To whom correspondence should be addressed.

The introduction and subsequent spread in the human population of influenza A viruses with a novel hemagglutinin (HA) or a novel HA and neuraminidase (NA) subtype results from a sudden and major change in virus antigenicity, which is referred to as an antigenic shift. Lack of protective immunity in the human population against the new HA and NA proteins can result in rapid global spread of the virus, leading to widespread morbidity and mortality. Pandemic strains contain new HA or NA genes derived from animal influenza A viruses. Influenza A viruses of 15 recognized HA subtypes and 9 NA subtypes are known to circulate in birds and other animals, creating a reservoir of influenza A virus genes available for genetic reassortment with circulating human strains of influenza virus. However, on the basis of seroarchaeology and virus isolation since 1933, only viruses of the H1, H2, and H3 subtypes are known to infect and cause disease in humans (Please register to see links). In general, avian influenza A viruses, including those that are highly pathogenic in birds, do not appear to replicate efficiently or cause disease in humans. The only reported natural infections of humans by avian viruses are two cases of conjunctivitis associated with avian H7 viruses, one of which was an infection with a seal virus of avian origin (Please register to see links). Serosurveys of farm workers in southern China by single radial hemolysis revealed a seroprevalence ranging from 1 to 38% for avian viruses of the H4 through H13 subtypes, including 7% seroprevalence for H5 viruses (Please register to see links). In contrast, there were no documented infections in U.S. poultry workers exposed to strains of avian (H5) influenza A viruses that were highly pathogenic in poultry (Please register to see links).
On 9 May 1997, a previously healthy 3-year-old boy, who was a resident of Hong Kong, developed a sore throat, dry cough, and fever. He was diagnosed with pharyngitis and prescribed antibiotics and aspirin. The child continued to be symptomatic and febrile and was hospitalized on 15 May. Upon admission, he was noted to be febrile (axillary temperature above 39°C) and irritable. His laboratory tests were most remarkable for leukopenia (2000 white blood cells per cubic millimeter). His chest x-ray was within normal limits. The next day, he was transferred to another hospital, where he developed progressive respiratory distress associated with hypoxemia, consistent with acute respiratory distress syndrome. He also became increasingly unresponsive. Computerized tomography of the head was unremarkable, and examination of his cerebrospinal fluid was not suggestive of an inflammatory process. Despite mechanical ventilation and broad antibiotic coverage, the child died on 21 May with several complications, including respiratory failure, renal failure, and disseminated intravascular coagulopathy. Postmortem liver and kidney biopsies showed evidence of microvascular fatty infiltration consistent with Reye's syndrome, which is a recognized complication of influenza.
A tracheal aspirate specimen was obtained on day 10 of illness and was cultured for respiratory viruses. A cytopathic effect was noted in mammalian Madin Darby canine kidney (MDCK) cells and rhesus monkey kidney (LLC-MK2) cells 2 and 3 days after inoculation, respectively. The isolate, designated A/Hong Kong/156/97, was typed as an influenza A virus by means of monoclonal antibodies to viral nucleoprotein in an immunofluorescence test, and it did not stain with monoclonal antibodies to respiratory syncytial virus and parainfluenza viruses 1, 2, and 3 (Please register to see links). Additionally, bacterial and fungal cultures of the tracheal secretions did not yield pathogenic organisms. The influenza A virus isolate was not inhibited by sheep hyperimmune antisera to H1 and H3 influenza viruses included in the World Health Organization's Influenza Reagent Kit. Confirmation of the initial identification of the isolate as influenza A was obtained by immunoperoxidase staining of infected MDCK monolayers with influenza virus-specific monoclonal antibodies (Please register to see links, Please register to see links). A hemagglutination-inhibition (HI) test with a panel of antisera to different subtypes of influenza A virus gave a reaction (HI titer 1280) only with the A/Tern/South Africa/61 (H5N3) antiserum, providing an identification of the HA as H5 (Please register to see links).
Because the antiviral drugs amantadine and rimantadine can be used for prophylaxis and treatment of influenza A virus infections, it was of interest to establish the antiviral sensitivity of this virus. Results of an in vitro assay revealed that the virus was sensitive to amantadine (Please register to see links).

Amplification of the HA and NA genes by polymerase chain reaction (PCR), from RNA extracted from the MDCK cell isolate, confirmed the HA subtype as H5 and identified the NA subtype as N1 (Please register to see links). Nucleotide sequence analysis of the HA gene (Fig. Please register to see links) revealed multiple basic amino acids at the cleavage site between the HA1 and HA2 domains, a feature (Please register to see links) that is associated with highly pathogenic H5 avian viruses and can be part of a motif (Arg-Lys-Lys-Arg) (Please register to see links). In addition, there are three basic amino acids in the four-amino-acid insertion (Arg-Gln-Arg-Arg) adjacent to the cleavage site. Phylogenetic analysis (Fig. Please register to see links) shows that there are two lineages of H5 HA genes: the Eurasian and North American (Please register to see links). The A/Hong Kong/156/97 virus lies in the Eurasian lineage. In addition to the sequence motif adjacent to the cleavage site, other features of the HA associated with virulence, such as glycosylation sites (Please register to see links), were conserved in the A/Hong Kong/156/97 virus. The residues of the H5 receptor binding site described by Garcia et al. are conserved in the A/Hong Kong/156/97 virus as well (Please register to see links). Fig. 1. Alignment and comparison of complete HA coding sequence for the A/Hong Kong/156/97 virus isolate. Dashes under the sequence adjacent to the cleavage site indicate the positions of the insertions relative to the reference strain A/Turkey/England/91. The boxed amino acids indicate potential glycosylation sites. Underlined, bold peptides describe the location of the receptor binding site (Please register to see links). The nucleotide sequence has been deposited in GenBank (accession number AF036356). Please register to see links

Fig. 2. Phylogenetic relationship of the HA1 domain of the hemagglutinin gene of representative avian H5 viruses and the H5 virus isolated from a human (A/Hong Kong/156/97, boxed). The tree is rooted in A/Chick/Scotland/59 (H5N1) as an outgroup. This is a neighbor-joining analysis determined by PHYLIP. Please register to see links







Sequence analysis of the NA gene of the A/Hong Kong/156/97 virus determined that the NA was of the N1 subtype, related most closely to the NA gene of the A/Parrot/Ulster/73 (H7N1) virus (Table Please register to see links). The stalk region, which extends from the viral membrane up to amino acid 85, had a 19-amino-acid deletion when aligned with other available N1 NA gene sequences. Large block deletions of 33 to 48 nucleotides have been described in the stalk region of N1 NA genes in several N1 subtype strains isolated between 1933 and 1957 (Please register to see links). The residues corresponding to those identified by Colman et al. (Please register to see links) surrounding the sialic acid binding site of the N2 NA (with the exception of 121Ile, which is seen in all N1 NA genes) are conserved in the A/Hong Kong/156/97 virus.
Table 1. Molecular analysis of the gene segments of the A/Hong Kong/156/97 virus. Nucleotide sequences were compared, and identity was determined by FASTA (Wisconsin Package, version 9.0) searches of GenBank and European Molecular Biology Laboratory (EMBL) databases as of August and June 1997, respectively. The nucleotide sequences have been deposited in GenBank (accession numbers AF036356 through AF036363).





Gene segmentRegion amplifiedRegion sequencedViruses with greatest degrees of identityIdentity (%)PB28-233761-2310A/Budgerigar/Hokkaido/1/77 (H4N6)90.1PB110-234032-2291A/Singapore/1/57 (H2N2)90.6PA1-220031-1754 and 1974-2179A/Duck/Hokkaido/8/80 (H3N8)91.0HA1-177321-1743A/Turkey/England/91 (H5N1)93.5NP7-156121-1542A/Mallard/Astrakhan/244/82 (H14N6)93.6NA1-140029-1391A/Parrot/Ulster/73 (H7N1)91.1M775-1027796-1001A/Turkey/Minnesota/833/80 (H1N1)98.5NS1-89027-860A/Duck/Hong Kong/717/79 (H1N3)93.7
Nucleotide sequence and phylogenetic analysis determined that genes encoding all internal and nonstructural proteins were most closely related to known genes of avian origin (Table Please register to see links). The similarity of the A/Hong Kong/156/97 PB1 gene to the corresponding gene of human A/Singapore/1/57 (H2N2) virus can be explained by the suggestion that, in 1957, this gene was transmitted to human influenza viruses from avian species (Please register to see links). The highest percent identities of the PB2, PA, NP, M, and NS genes of A/Hong Kong/156/97 to human influenza virus genes were 83.3, 83.6, 85.4, 91.2, and 89.3%, respectively, 3.1 to 9.9% lower than the percent identity with avian viruses listed in Table Please register to see links. Therefore, all gene segments of the A/Hong Kong/156/97 virus appeared to be avian in origin with no evidence of genetic reassortment with recent human influenza A virus genes.
To determine whether the A/Hong Kong/156/97 virus isolated in MDCK cells retained pathogenicity for avian species, we inoculated the virus by intravenous and intranasal routes into 16 3-week-old White Plymouth Rock chickens (Gallus domesticus) and by combined intranasal and intratracheal routes into six adult female White Leghorn chickens. The virus killed all eight chickens inoculated intravenously within 3 days; all of the chickens inoculated intranasally or by combined routes died within 9 days, except one intranasally inoculated White Plymouth Rock chicken, which survived. The chickens that died had lesions consistent with highly pathogenic avian influenza (fowl plague) and included necrosis of the combs and subcutaneous hemorrhages of the legs, thoracic wall, fat pads, proventriculus, and serosa of the heart. Most chickens had moderate to severe diffuse interstitial pneumonia with demonstration of influenza viral antigen in blood vessel and air capillary endothelium and macrophages. The virus also replicated in other tissues, as indicated by the presence of viral antigen in neurons, cardiac myocytes, and blood capillary endothelial cells. The A/Hong Kong/156/97 virus remained highly pathogenic for birds after replication in mammalian cells.

To rule out laboratory contamination as a source of the isolate, we confirmed that the H5N1 virus could be identified directly in the tracheal aspirate obtained from the patient. The MDCK cells and embryonated eggs re-inoculated with the original clinical material yielded only an H5 virus. In addition, successful PCR amplification of the HA gene from RNA extracted from the clinical material was achieved with only H5 HA-specific primers and not with H1, H3, or B HA-specific primers (Fig. Please register to see links). The nucleotide sequences of the coding regions of the HA and NA genes amplified from RNA extracted from the clinical material were identical to the sequences determined for the virus isolated in MDCK cells (Please register to see links). Cells from the clinical material were stained by immunofluorescence with monoclonal antibodies against the H5 HA and influenza A NP but not with monoclonal antibody against the H3 HA and a pool of influenza B-specific monoclonal antibodies (Fig. Please register to see links). Paired sera from the patient were not available; however, a single serum specimen collected on day 10 of illness did not react with the isolate in an HI test. Fig. 3. Amplification of influenza HA gene from RNA extracted directly from the tracheal aspirate obtained from the patient. Lanes 1 and 14 contain a DNA ladder, with fragment sizes indicated along the left edge of the gel. RNA was extracted by means of the Qiagen (Valencia, California) RNeasy kit from the clinical material (lanes labeled HK/156/97: 4, 7, 10, 13), from known H1 (lane 2), H3 (lane 5), and H5 (lane 8) influenza A viruses, influenza B virus (lane 11), and water controls (lanes labeled : 3, 6, 9, 12); reverse transcriptase reactions were carried out using 5 µl of RNA and 25 units of avian myeloblastosis virus reverse transcriptase in a 25-µl reaction. PCR was carried out using H1 (lanes 2 to 4), H3 (lanes 5 to 7), H5 (lanes 8 to 10), and B (lanes 11 to 13) HA-specific primers (PP, primer pairs); the expected product sizes are 1104, 785, 1110, and 1104 base pairs, respectively. PCR cycling conditions included denaturation at 94°C for 7 min, annealing for 2 min at 50°C, and extension for 3 min at 72°C, followed by 29 cycles of denaturation at 94°C for 1 min, annealing for 2 min at 50°C, extension for 3 min at 72°C, and an additional extension of 7 min at 72°C. Please register to see links
Fig. 4. Immunofluorescence staining of cells from the tracheal aspirate obtained from the patient. Acetone-fixed cells were incubated with influenza virus-specific monoclonal antibodies followed by fluorescein isothiocyanate-conjugated goat antibodies to mouse immunoglobulin G. The influenza virus-specific monoclonal antibodies used were (A) a pool of two antibodies raised against the H5 HA of A/chicken/Pennsylvania/1370/83, (B) an antibody that is broadly cross-reactive with the HA of human H3N2 viruses, (C) a pool of two antibodies specific for the NP of influenza A viruses, and (D) antibody specific for influenza B viruses. Please register to see links

Although a clear epidemiologic link was not established between the infected child and infected poultry, there were three outbreaks of influenza in poultry on farms in Hong Kong between late March and early May 1997. Two viruses from one of these outbreaks were H5N1 viruses. Additionally, there were a few sick chickens at the child's preschool, but there is no evidence that the chickens were infected with avian influenza or that the child was in close contact with them.
The HA is the main determinant of the host range of influenza A viruses, a phenotype conferred by its specificity for receptor recognition and binding. All key amino acid residues believed to be associated with the specificity of receptor binding of H5 HA (Please register to see links) were conserved in the A/Hong Kong/156/97 virus. Identification of the specific linkages (2,6 or 2,3 Gal) that can be bound by the A/Hong Kong/156/97 virus and knowledge of the distribution of receptors bearing these linkages in the human respiratory tract may help explain why this avian H5 virus infected this child.
In addition to its role as a determinant of host range, the HA gene is important in determining the virulence of avian influenza A viruses for poultry (Please register to see links). All human influenza virus HA genes characterized to date, including the 1918 viruses (Please register to see links), have a single, basic Arg residue at the cleavage site between HA1 and HA2. An insertion similar to the basic amino acid insertion adjacent to the cleavage site in the HA gene of the A/Hong Kong/156/97 virus has been found in several highly pathogenic avian H5 viruses. Basic amino acids adjacent to the cleavage site allow proteases other than trypsin-like proteases to cleave the HA into HA1 and HA2 domains. This event would enable the virus to spread systemically by altering the tissue range of these viruses from the respiratory and alimentary tract to other sites, such as the brain, heart, and blood vessels (Please register to see links). It has been suggested that the acquisition of a ubiquitously cleavable avian HA by a human influenza A virus could make a virus lethal (Please register to see links). It remains to be shown whether gene segments other than the HA may also have contributed to the ability of the virus to infect a human.
A pandemic of influenza could begin with isolated cases, in which avian or swine influenza viruses adapt to human hosts or, over time, genetically reassort with circulating human influenza A viruses, or it could begin as a rapid and explosive spread of a pandemic virus derived from a reassortment event in an intermediate host. Increased surveillance efforts have been initiated to identify other cases of illness associated with influenza A (H5N1) viruses. In addition, serosurveys are under way in an effort to identify asymptomatic or mild clinical infections in the region and substantiate the previous report of seroprevalence to H5 viruses (Please register to see links). These studies may determine whether H5 viruses similar to A/Hong Kong/156/97 are circulating in the human population and if the isolation of the A/Hong Kong/156/97 virus is the first step in the recognition of an influenza A virus with pandemic potential or whether this case is simply an isolated event.
Note added in proof: Since the submission of this report, there have been 12 additional confirmed human cases of influenza A (H5N1) infections in Hong Kong, including three fatalities. Sequence analysis of the genes of six of the isolates revealed that all of the genes are of avian origin and are closely related to each other. The HA gene codes for a multiple basic amino acid insertion upstream of the cleavage site, associated with highly pathogenic avian influenza viruses and identical to that seen in the A/Hong Kong/156/97 virus.
REFERENCES AND NOTES
  1. B. R. Murphy and R. G. Webster, in Field's Virology, B. N. Fields et al., Eds. (Lippincott Raven, Philadelphia, 1996), pp. 1397-1445.
  2. R. G. Webster, J. Geraci, G. Petursson, K. Skirnisson, N. Engl. J. Med. 304, 911 (1981) Please register to see linksPlease register to see links ; J. Kurtz, R. J. Manvell, J. Banks, Lancet 348, 901 (1996) Please register to see linksPlease register to see linksPlease register to see links .
  3. K. F. Shortridge, Semin. Respir. Infect. 7, 11 (1992) Please register to see links .
  4. W. J. Bean, Y. Kawaoka, J. M. Wood, J. E. Pearson, R. G. Webster, J. Virol. 54, 151 (1985) Please register to see links .
  5. K. Subbarao et al., unpublished data.
  6. T. Ziegler, H. Hall, A. Sanchez-Fauquier, W. C. Gamble, N. J. Cox, J. Clin. Microbiol. 33, 318 (1995) Please register to see links .
  7. With antisera raised in animals to H1 through H8 and H10 through H13 influenza A viruses, the HI titers for the A/Hong Kong/156/97 virus were <10, except for antiserum to A/Tern/South Africa/61 (H5N3), which had a titer of 1280.
  8. G. W. Wood, J. Banks, J. W. McCauley, D. J. Alexander, Arch. Virol. 134, 185 (1994) Please register to see linksPlease register to see linksPlease register to see links .
  9. D. A. Senne, et al., Avian Dis. 40, 425 (1996) Please register to see linksPlease register to see linksPlease register to see links .
  10. C. Rohm, T. Horimoto, Y. Kawaoka, J. Suss, R. G. Webster, Virology 209, 664 (1995) Please register to see linksPlease register to see linksPlease register to see links .
  11. Y. Kawaoka, C. W. Naeve, R. G. Webster, ibid. 139, 303 (1984) Please register to see linksPlease register to see linksPlease register to see links.
  12. M. Garcia, J. M. Crawford, J. W. Latimer, E. Rivera-Cruz, M. L. Perdue, J. Gen. Virol. 77, 1493 (1996) Please register to see links .
  13. J. Blok and G. M. Air, Virology 118, 229 (1982) Please register to see linksPlease register to see linksPlease register to see links ; M. C. Els, G. M. Air, K. G. Murti, R. G. Webster, W. G. Laver, ibid. 142, 241 (1985) Please register to see linksPlease register to see linksPlease register to see links.
  14. P. M. Colman, J. N. Varghese, W. G. Laver, Nature 303, 41 (1983) Please register to see linksPlease register to see links .
  15. Y. Kawaoka, S. Krauss, R. G. Webster, J. Virol. 63, 4603 (1989) Please register to see links .
  16. Y. Kawaoka and R. G. Webster, Microb. Pathog. 5, 311 (1988) Please register to see linksPlease register to see linksPlease register to see links .
  17. J. K. Taubenberger, A. H. Reid, A. E. Krafft, K. E. Bijwaard, T. G. Fanning, Science 275, 1793 (1997) Please register to see links .
  18. R. G. Webster and R. Rott, Cell 50, 665 (1987) Please register to see linksPlease register to see linksPlease register to see links ; I. P. Mo, M. Brugh, O. J. Fletcher, G. N. Rowland, D. E. Swayne, Avian Dis. 41, 125 (1997) Please register to see linksPlease register to see linksPlease register to see links ; C. C. Brown, H. J. Olander, D. A. Senne, J. Comp. Pathol. 107, 341 (1992) Please register to see linksPlease register to see linksPlease register to see links .
  19. We gratefully acknowledge the contributions of K. Shu-wing, J. Chan, and the doctors and nurses of Queen Elizabeth Hospital, Hong Kong; P. Lo and the doctors at the Baptist Hospital, Hong Kong; M. F. C. Chan, P. T. Saw, K. H. Mak, and T. H. Tsang of the Hong Kong Department of Health; J. S. Tam of Chinese University of Hong Kong; K. F. Shortridge of the University of Hong Kong; K. Liu of the Department of Agriculture and Fisheries, Hong Kong; D. Suarez of the Southeast Poultry Research Laboratory, Athens, GA; and H. Izurieta, C. Dentinger, T. Voss, G. Perez-Oronoz, J. Love, W. Gamble, and I. Baker of the Centers for Disease Control and Prevention, Atlanta, GA. We thank R. G. Webster of St. Jude Children's Research Hospital, Memphis, TN, for sharing oligonucleotide primers for and monoclonal antibodies against the H5 HA, and E. Claas and A. Osterhaus of Erasmus University, Rotterdam, Netherlands, for sharing primer sequences.
26 September 1997; accepted 9 December 1997
Last edited by quandary121; Jul 2nd, 2008 at 12:32 PM.
Tonington
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  #27
Jul 2nd, 2008
Quoting quandary121
I'm not disputing this but don't you think that to engineer DNA in a flu vaccine is dangerous.????
No, it's done all the time. Making vaccines is bioengineering. If they were inoculating people with live fully functional viruses, then I would be worried. But that would be stupid, and of course that's not what they're doing.
quandary121
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lincolnshire
  #28
Jul 2nd, 2008
Quoting Tonington
No, it's done all the time. Making vaccines is bioengineering. If they were inoculating people with live fully functional viruses, then I would be worried. But that would be stupid, and of course that's not what they're doing.
SO THE DOCTOR SITED IN THIS VIDEO IS WRONG TO SCARE US IN THIS WAY IS THAT WHAT YOUR SAYING


URGENT WARNING: H5N1 DNA in Flu Vaccine
Tonington
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  #29
Jul 2nd, 2008
Quoting quandary121
SO THE DOCTOR SITED IN THIS VIDEO IS WRONG TO SCARE US IN THIS WAY IS THAT WHAT YOUR SAYING
Yes, that is what I'm saying. Dr. Laibow practices drug free medicine, and so as part of her position at Natural Solutions Foundation, she campaigns against drugs.

It's unlikely that a pandemic can be stopped. The turnaround from known threat to viable vaccine is not a large window. But how do you think that could be done with natural medicine? Nutrition is key to her foundations successes, and that's great. But with so many countries with poor malnourished people, it's also highly unlikely. The threat will likely emerge from Asia, where there are many poor subsistence farmers who live with their flocks of birds and their feces in close quarters.

If you have a highly pathogenic outbreak in Asia, and it mutates in the human host after leaving the avian vector, nutrition won't help at all. Vaccinations might have a shot, but it's still a Hail Mary. You have to isolate the specific strain of H5N1 in the early stages and then mass produce a vaccine, very quickly. There's only three companies worldwide which have developed the technology to do this.

Having a vaccine now with H5N1 DNA won't likely help, because there is no pandemic, the markers won't likely be correct due to the high mutation rate. But having a vaccine with H5N1 DNA also will not increase the likelihood of a pandemic. It just doesn't work that way. There are dozens of the 'highly pathogenic variety', and many more of the 'low pathogenic variety'.

What you aren't getting, is that this isn't a live complete virus. It has DNA from some strain of H5N1, but it isn't a complete virus. The virus we're concerned about, needs all 8 strands of RNA in order to replicate in the host cells. The vaccine doesn't require DNA that allows a virus to replicate, only the DNA which codes for surface proteins, which is how your leukocytes recognize foreign objects, and make the antibodies.

Just because someone with some letters behind their name says you should be concerned, doesn't mean they're correct. Where is her research? There's libraries of supporting research worldwide that supports vaccination programs. The letters behind the name are an appeal to authority without the actual data and statistics to back up what she says.

This is much ado about nothing.
quandary121
Time Out
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2,950 Posts
lincolnshire
  #30
Jul 2nd, 2008
Quoting Tonington
Yes, that is what I'm saying. Dr. Laibow practices drug free medicine, and so as part of her position at Natural Solutions Foundation, she campaigns against drugs.

It's unlikely that a pandemic can be stopped. The turnaround from known threat to viable vaccine is not a large window. But how do you think that could be done with natural medicine? Nutrition is key to her foundations successes, and that's great. But with so many countries with poor malnourished people, it's also highly unlikely. The threat will likely emerge from Asia, where there are many poor subsistence farmers who live with their flocks of birds and their feces in close quarters.

If you have a highly pathogenic outbreak in Asia, and it mutates in the human host after leaving the avian vector, nutrition won't help at all. Vaccinations might have a shot, but it's still a Hail Mary. You have to isolate the specific strain of H5N1 in the early stages and then mass produce a vaccine, very quickly. There's only three companies worldwide which have developed the technology to do this.

Having a vaccine now with H5N1 DNA won't likely help, because there is no pandemic, the markers won't likely be correct due to the high mutation rate. But having a vaccine with H5N1 DNA also will not increase the likelihood of a pandemic. It just doesn't work that way. There are dozens of the 'highly pathogenic variety', and many more of the 'low pathogenic variety'.

What you aren't getting, is that this isn't a live complete virus. It has DNA from some strain of H5N1, but it isn't a complete virus. The virus we're concerned about, needs all 8 strands of RNA in order to replicate in the host cells. The vaccine doesn't require DNA that allows a virus to replicate, only the DNA which codes for surface proteins, which is how your leukocytes recognize foreign objects, and make the antibodies.

Just because someone with some letters behind their name says you should be concerned, doesn't mean they're correct. Where is her research? There's libraries of supporting research worldwide that supports vaccination programs. The letters behind the name are an appeal to authority without the actual data and statistics to back up what she says.

This is much ado about nothing.
well thanks Tonington for pointing this out to me, i will consider this thread close thanks to you wonderful tutoring, thanks for sticking with it, i was concerned and maybe i jumped the gun here,

case closed



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